Mushrooms are members of a specific biotic group, somewhere midway between plants and animals—fungi.

A total of 233 species of macrofungi have been identified within Rila National Park, making up 11.6% of all mushroom species in Bulgaria, and half of those known in the Rila Mountains as a whole. One species of those growing in the Park, Albatrellus cristatus, is protected in Europe.

Eleven species of edible mushrooms have been identified that may be of interest to commercial gatherers:

• Agaricus silvaticus Schaeff.: Fr.;
• Boletus edulis Bull.:Fr.;
• Boletus pinophyllus Pilat & Dermek;
• Cantharellus cibarius Fr.;
• Hydnum repandum L.;
• Lactarius deliciosus (L.: Fr.) S. F. Gray;
• Lepista nuda (Bull.: Fr.) Cooke;
• Ramaria flava (Schaeff.: Fr.) Quel.;
• Sarcodon umbricatus (Fr.) Karst.;
• Suillus luteus (L.) S. F. Gray;
• Xerocomus badius (Fr.) Kuhner: Gilb.